Isolation of three thioredoxins from spinach leaves.
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چکیده
منابع مشابه
Isolation of dihydroxyacetone phosphate reductase from dunaliella chloroplasts and comparison with isozymes from spinach leaves.
A dihydroxyacetone phosphate (DHAP) reductase has been isolated in 50% yield from Dunaliella tertiolecta by rapid chromatography on diethylaminoethyl cellulose. The activity was located in the chloroplasts. The enzyme was cold labile, but if stored with 2 molar glycerol, most of the activity was restored at 30 degrees C after 20 minutes. The spinach (Spinacia oleracea L.) reductase isoforms wer...
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The properties of two amylase activities which differ in their substrate specificity and subcellular location as well as a chloroplast-associated R-enzyme (debranching activity) are reported. An extrachloroplastic amylase is resolved by gel filtration chromatography into two activities of 80,000 and 40,000 daltons. Both extrachloroplastic activities hydrolyze amylopectin and shellfish glycogen ...
متن کاملAminotransferases in peroxisomes from spinach leaves.
Organelles in spinach leaves were isolated in sucrose gradients by isopycnic centrifugation. The peroxisomal fraction was the only cellular site for two different and irreversible aminotransferases which utilized glyoxylate as the amino acceptor in the formation of glycine. These were a serine:glyoxylate aminotransferase with a specific activity of 1.54 pmoles X min-1 X mg-1 peroxisomal protein...
متن کاملIsolation and oxidative properties of intact mitochondria isolated from spinach leaves.
A procedure was described for preparing intact mitochondria from spinach (Spinacia oleracea L.) leaves. These mitochondria oxidized succinate, malate, pyruvate, alpha-ketoglutarate, and NADH with good respiratory control and ADP/O ratios comparable to those observed with mitochondria from other plant tissues. Glycine was oxidized by the preparations. This oxidation linked to the mitochondrial e...
متن کاملIsolation of photosystem I particles from spinach.
A method to prepare photosystem I (PSI) particles is described. Spinach leaves are used to prepare broken chloroplasts that are then solubilized by using a detergent (Triton X-100). Solubilized chloroplasts are then applied on an ion-exchange column. Eluted by a linear concentration gradient of NaCl, fractions enriched in PSI particles are collected and applied on a small hydroxyapatite column....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37818-3